Figure 6.

L. bicolor root components (LBR) suppress BT549 and MDA-MB-231 migration by regulating F-actin expression and focal adhesion kinase (FAK) inactivation. A-D. Cells were treated with 0.01% dimethyl sulfoxide (control) or LBR at 5, 10, and 20 μg/mL for 48 h and the phosphorylation levels of FAK were determined by western blotting. E, G. Immunofluorescence staining of 4’,6-diamidino-2-phenylindole (DAPI) (blue), anti-FAK antibody (green), and phalloidin (red) in BT549 and MDA-MB-231 cells. F, H. Quantification of the FA surface area. Values indicate the average ± standard deviation from three independent experiments. *P < 0.05 and **P < 0.01 compared with the control group.