Figure 8.

Evaluation of acute toxicity of Lespedeza bicolor root components (LBR) in mice. Six to eight-week-old male BALB/c-nu nude mice were treated with LBR (100 mg/kg and 300 mg/kg) or the saline control. A, B. Serum levels of liver injury markers alanine transferase (ALT), aspartate aminotransferase (AST), and creatinine (Cr) were measured. C. Liver tissues were stained with hematoxylin and eosin (H&E) for histological analysis. D. Body weight was monitored throughout the treatment period. E-I. Changes in heart, liver, lung, kidney, and spleen weight were assessed during LBR treatment. Data are represented as mean ± standard deviation (SD) (n = 6); *P < 0.05 and **P < 0.01 compared with the control group.