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. 2024 Sep 15;14(9):4429–4444. doi: 10.62347/KXJJ8824

Figure 2.

Figure 2

WTp53 increases the expression of PUMA, BAX, and p21 in ATC cells. (A) Comparison of the expression of the PUMA and BAX genes at the mRNA by RT-qPCR analysis in 8505C, 8505C-WTp53, and 8505C-MTp53 cells respectively (n=3). (B) Comparison of protein levels of PUMA and BAX in 8505C, 8505C-WTp53, and 8505C-MTp53 cells by IHC analysis [B-I, images; B-II, quantitation of cell positively immuno-stained with PUMA (d-f of B-I) or BAX (g-i of B-I)]. (C-I) In ATC cells, p53 expression increases the expression of p21 at the mRNA and protein levels. (C-II) Representative western blot analysis showing high expression of p53 and p21 proteins in ATC cells (II-ii, n=3). (C-III) Representative IHC images (i) and quantitative analysis of IHC results (ii, n=3) for p21 (d-f). Significant differences are indicated by asterisks (*P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001). Data represent the mean ± SD (n=3).