Figure 4.

Expression of WTp53 attenuates oncogenic actions of mutant p53 in 8505C cells. A-I. Gene Set Enrichment Analysis of scRNA-seq identified top pathways contributing to counteracting oncogenic actions of mutant p53. A-II. Gene enrichment plot of TNFα signaling via NFkB analyzed by differential expression genes between WTp53 and 8505C cells. B. Validation of the top 11 genes in mediating signaling TNFα/NFkB by q/PCR by comparing the extent of mRNA expression of the 11 genes as shown. The gene names are as listed. Significant differences are indicated by asterisks (*P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001). Data represent the mean ± SD (n=3).