Table 2.
Assay | Normal Control | Negative Control | Concentration of Test Substance (µg/mL) | Concentration of Positive Control (µg/mL) |
||
---|---|---|---|---|---|---|
1 μg/mL | 4 μg/mL | 16 μg/mL | ||||
SOD activity (%) | 100 ± 0.0 | 90.6 ± 7.9 | 93.5 ± 1.4 | 142.1 ± 14.1 | 107.4 ± 10.2 | 170.5 ± 59.9 |
GST activity (%) | 100 ± 0.0 | 82.8 ± 0.1 ** | 109.9 ± 2.9 $$ | 99.5 ± 0.8 $$ | 119.6 ± 4.1 $$ | 108.2 ± 3.2 $$ |
ALT concentration (pg/mL) | 712.9 ± 7.8 | 728.1 ± 16.0 | 707.4 ± 8.5 | 679.8 ± 16.7 | 697.7 ± 8.9 | 674.3 ± 18.6 |
AST concentration (pg/mL) | 155.1 ± 2.4 | 198.5 ± 11.8 ** | 181.1 ± 4.7 a | 171.5 ± 3.6 aa | 155.1 ± 2.4 aa | 157.0 ± 5.9 aa |
Oil Red O staining assay (OD values at 510 nm) |
0.150 ± 0.009 | 0.221 ± 0.009 ** | 0.190 ± 0.001 $$ | 0.159 ± 0.014 $$ | 0.141 ± 0.009 $$ | 0.044 ± 0.001 $$ |
Data are presented as the mean ± SE. The results were statistically analyzed using Student’s t-test or one-way ANOVA. a p < 0.05, significant difference compared to the negative control; Dunnett’s t-test. aa p < 0.01, significant difference compared to the negative control; Dunnett’s t-test. ** p < 0.01, significant difference compared to the normal control; Student’s t-test. $$ p < 0.01, significant difference compared to the negative control; Welch’s t-test.