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. 2024 Sep 28;16(19):3297. doi: 10.3390/nu16193297

Figure 1.

Figure 1

In vivo study design and group allocation. The flowchart illustrates the design of our in vivo study involving adult female Sprague-Dawley (SD) rats. The rats were divided into six groups (n = 7 per group): (1) non-ovariectomized control (Sham); (2) ovariectomized control (OVX); (3) positive control group receiving 0.1 mg/kg of 17β-estradiol (E2); groups 4–6 were OVX groups treated with the blended SJF–GSE complex extract at 25 mg/kg (Low), 50 mg/kg (Medium), and 100 mg/kg (High), respectively. After the 3-week recovery period, the rats were provided with custom-manufactured diets containing the specific treatment compounds. The SJF-GSE complex extract was prepared as detailed in the Section 2, with a 1.5:1 ratio of SJF to GSE. SJF, Styphnolobium japonicum fruit; GSE, germinated soybean embryo.