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. 2024 Sep 28;16(19):3297. doi: 10.3390/nu16193297

Figure 2.

Figure 2

Evaluation of the estrogen-like activity of Styphnolobium japonicum fruit extract (SJFE) and germinated soybean embryo extract (GSEE) combinations in MCF7 cells. (A) Expression of pS2 mRNA, quantified using qPCR. (B–D) Representative immunoblots of ERα, phosphorylated Akt (p-Akt), total Akt, and β-actin. (E) Immunocytochemical analysis of ERα expression (left panel). The relative ERα/PI ratio is presented as fold-change relative to the control (right panel). 17β-estradiol (E2) was used as a positive control, and ICI 182,780 was used as an estrogen antagonist. Scale bar, 100 µm. Results from three independent experiments are shown as means ± standard deviation. * p < 0.05, ** p < 0.01, vs. the control group; ## p < 0.01, vs. the indicated group. ER, estrogen receptor; PI, propidium iodide.