Fig 2. Seizures occur spontaneously in mice with Phf6 deletion through a neural intrinsic mechanism.

(A) Time to first seizure in female heterozygous germline deleted Phf6^+/– mice compared to Phf6^+/+ wild-type control mice. Wild-type mice did not display seizures. Median time to seizure onset was 662 days. N = 70 Phf6^+/+ mice and 103 Phf6^+/– mice. (B) Time to first seizure in females with Phf6 heterozygous deletion in the nervous system compared to controls; Phf6^+/+;Nestin-cre transgenic control mice did not display seizures. N = 11 Phf6^+/+;Nes-cre^Tg/+ and 11 Phf6^lox/+;Nes-cre^Tg/+ mice. Time to seizure onset of a small number of Phf6^lox/lox;Nes-cre^Tg/+ mice (homozygous nervous system-specific deleted) is shown in S1 Fig. (C) Time to first seizure in males with Phf6 deletion in the nervous system compared to controls. Phf6^+/Y;Nestin-cre transgenic control mice did not display seizures. N = 33 Phf6^+/Y;Nes-cre^Tg/+ and 13 Phf6^lox/Y;Nes-cre^Tg/+ mice. Checked points indicate mice that were sacrificed due to illness or other reasons. Data were analysed by a log-rank (Mantel-Cox) test. NS = nervous system. Survival data of mice with germline deletion of Phf6 are shown in S2 Table.