Table 2.
Chromatographic and mass parameters for the BMAA and the detection of isomers AEG and DAB.
| Target | Retention Time (min) | Derivatized Ion Precursor (m/z) | CID Fragments (m/z) | CID Colission Energy (V) | Calibration: Curve and Linear Interval (µg/L) |
LOD (µg/L) |
LOQ (µg/L) |
|---|---|---|---|---|---|---|---|
| BMAA | 13.92 ± 0.08 | 459.00 | 320, 315, 289, 258, 171, 145 | 35 | y = 1377.4x − 2154.1 r2 = 0.9947 3-250 |
7.99 | 24.2 |
| AEG | 13.22 ± 0.11 | 459.00 | 320, 315, 289, 272, 171, 145 | 35 | y = 889.91x + 4972.2 r2 = 0.9993 3-150 |
57.4 | 174 |
| 2,4-DAB | 14.93 ± 0.15 | 459.00 | 320, 315, 289, 271, 171, 145 | 35 | y = 2785x + 5538.1 r2 = 0.9934 7-250 |
70 | 212 |
LOD—limit of detection; LOQ—limit of quantification; CID—collision-induced dissociation.