Table 4.

Amplification conditions for the cyanotoxic genes in Azolla and N. azollae DNA.

Gene	Initial Denaturation	Denaturation	Annealing	Extension	Final Extension	Reference
sxt	94 °C; 3 min	35 cycles			72 °C; 7 min	[57]
		94 °C; 10 s	52 °C; 20 s	72 °C; 1 min
cyl	94 °C; 10 min	30 cycles			72 °C; 7 min	[59]
		94 °C; 30 s	55 °C; 30 s	72 °C; 7 min
mcyE/ndaF	92 °C; 2 min	35 cycles			72 °C; 5 min	[60]
		92 °C; 20 s	56 °C; 30 s	72 °C; 1 min
mcy A	95 °C; 2 min	35 cycles			72 °C; 7 min	[61]
		95 °C; 90 s	56 °C; 30 s	72 °C; 50 s
mcy B	94 °C; 2 min	35 cycles			72 °C; 5 min	[62]
		94 °C; 30 s	59 °C; 45 s	72 °C; 1 min
mcy C	94 °C; 2 min	35 cycles			72 °C; 7 min	[63]
		94 °C; 10 s	50 °C; 20 s	72 °C; 1 min
mcy B, domain A	94 °C; 4 min	30 cycles			72 °C; 7 min	[64]
		95 °C; 30 s	52 °C; 30 s	72 °C; 1 min
mcy C, domain A	94 °C; 5 min	35 cycles			72 °C; 7 min	[65]
		95 °C; 1 min	52 °C; 30 s	72 °C; 1 min
mcy D, domain ACP	94 °C; 5 min	35 cycles			72 °C; 7 min	[65]
		95 °C; 1 min	52 °C; 30 s	72 °C; 1 min
mcy D, domain KS	94 °C; 5 min	35 cycles			72 °C; 7 min	[65]
		95 °C; 1 min	52 °C; 30 s	72 °C; 1 min
mcy E, domain GST-AMT	94 °C; 5 min	35 cycles			72 °C; 7 min	[65]
		95 °C; 1 min	52 °C; 30 s	72 °C; 1 min
mcy G, domain CM	94 °C; 5 min	35 cycles			72 °C; 7 min	[65]
		95 °C; 1 min	52 °C; 30 s	72 °C; 1 min