FIG. 8.
Impact of the UGACC→UGAACC mutation on the RNA-binding activity of NSP3. Complexes formed by incubating purified rNSP3 with the 32P-labeled RNA probes v40-GACC and v40Δ3′-11 (A) or v40-GACC and v40-GAACC (B) were resolved by electrophoresis on a nondenaturing 6% polyacrylamide gel and detected by autoradiography. The intensity of bands representing rNSP3-probe complexes and free probe were quantified with a PhosphorImager. The values were used to calculate the percentage of probe binding to rNSP3.