FIG. 9.

Effect of the UGACC→UGAACC mutation on protein expression in infected cells. The chimeric reporter RNAs g6-Fluc-UGACC and -UGAACC and glo/g6-Fluc-UGACC and -UGAACC were separately cotransfected with nv-Rluc into SA11-infected MA104 cells at 1 h p.i. At 9 h p.i., the levels of firefly and Renilla luciferases per milligram of cell lysate were determined and the expression of firefly luciferase was normalized to the expression of Renilla luciferase. To ease the comparison of values, the expression of firefly luciferase for the g6-Fluc-UGACC and glo/g6-Fluc-UGACC RNAs was set to 100%.