TABLE 1.
T-cell FACS analysisa
| Sample | % CD3+ CD4+
|
% CD3+ CD8+
|
||
|---|---|---|---|---|
| Day 4 | Day 5 | Day 4 | Day 5 | |
| Acutely challenged | ||||
| C57 | 1.80 ± 0.34 | ND | 1.25 ± 0.16 | ND |
| GKO | 2.54 ± 0.36 | ND | 1.91 ± 0.24 | ND |
| Immunized and challenged | ||||
| C57 | 6.10 ± 2.291,5 | 8.34 ± 3.281,6 | 5.79 ± 3.362,7 | 11.12 ± 3.352,8 |
| GKO | 7.13 ± 1.943,5 | 13.22 ± 5.673,6 | 5.05 ± 2.304,7 | 17.97 ± 5.494,8 |
a
Percentages of CD3+ CD4+ and CD3+ CD8+ cells out of the total number of cells analyzed are shown. The values represent the mean ± the standard error of the mean. For acutely challenged mice, a total of 10 mice were individually analyzed for each strain. For immunized and challenged mice, a total of 15 normal mice and 13 GKO mice were individually analyzed in separate experiments. ND, samples not tested. Statistical significance is indicated by superior numbers as follows: 1, 5, 6, and 7, not significant; 2 and 4, P < 0.01; 3 and 8, P < 0.05.