Table 1.

Conditions and time points for human amniotic mesenchymal stem cell culture and passaging.

Cell	Culture conditions		Time points
Primary Cells(P0)	T75 Flask, 95% Air and 5% CO2, 37 °C		Day1
First-Passage Cells(P1)	T25 Flask, 95% Air and 5% CO2, 37 °C		Day7
Second-Passage Cells(P2)	T25 Flask, 95% Air and 5% CO2, 37 °C		Day12
Third-Passage Cells(P3)	T25 Flask, 95% Air and 5% CO2, 37 °C		Day16