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. 2024 Oct 10;13:285. doi: 10.1038/s41377-024-01612-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–f Phalloidin-alexa488 labeling of the actin cytoskeleton on a cultured cell imaged with EDF-RIM (a), EDF-WF (b) and EDF-WF deconvolved (c). The projected depth is 5.5 μm. Insets show a close-up view of the outlined region (d–f). Scale bar of the full image (a) and of the outlined region (d) are respectively 10 μm and 1 μm. g Line profiles along the segments shown in EDF-RIM (d) and deconvolved EDF-WF (f). EDF-RIM is able to distinguishes two filaments separated by 155 nm (lines 2,5) whereas deconvolved EDF-WF distinguishes at best two filaments separated by 269 nm (line 4)