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. 2024 Oct 10;13:285. doi: 10.1038/s41377-024-01612-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Image of desmosomes from the intestinal mouse epithelium with EDF-RIM, in which the entire 5.4 μm depth is acquired simultaneously. b, c Two slices (z = 3, and z = 11) of the same tissue imaged with 2D-RIM. The volume acquisition required 14 sequential z-slices. d Close-up view on the EDF-RIM image. e, f Close-up view on 2D-RIM at planes z = 2 and z = 7. Red and blue arrows point to structures in the EDF-RIM image that are only visible in one of the 2D-RIM images. Scale bar of the full image (a) and of the outlined region (d) are respectively 10 μm and 1 μm