FIG. 2.

Detection of recombinant HBsAg in cell culture fluid. HuH-7 cells were cotransfected with plasmids encoding one of the HBsAg proteins, a plasmid encoding L-HDAg (see Fig. 3), and pSEAP. Supernatants were harvested, and HBsAg was measured by a chemiluminescence assay. The light counts were normalized by an SEAP assay.