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Equilibrium density gradient analysis of HBsAg VLPs isolated from cell culture fluid. The VLPs were centrifuged through a 20% sucrose cushion, resuspended in PBS, and then centrifuged to equilibrium on preformed step gradients of CsCl (10 to 40% [wt/wt]). (A) Wild-type (wt) HBsAg particles. (B) Recombinant particles expressing HVR1-1b.
