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. 2001 Mar;75(5):2174–2184. doi: 10.1128/JVI.75.5.2174-2184.2001

FIG. 2.

FIG. 2

FIG. 2

cis-acting elements in the TBLV LTR lead to high transcriptional activity in T cells. (A) Reporter gene constructs used in transient transfection assays. The positions of relevant restriction enzyme sites within the U3 region that were used for cloning are shown. The construct pTBLV-LUC was prepared by substituting the ClaI-to-SstI fragment of TBLV for the ClaI-to-SstI fragment of pC3H-LUC. The construct pC3H3RΔNRE-LUC was made by substitution of a StuI-to-SstI fragment from the TBLV LTR for the StuI-to-SstI fragment of pC3H-LUC. (B) Activities of reporter gene constructs in Jurkat cells. Luciferase (LUC) activity is given in light units/100 μg of protein normalized for DNA uptake as measured by cotransfection with the pRL-TK reporter plasmid. Luciferase activity is reported relative to that of pC3H-LUC, assigned a value of 1; standard deviations from the means of triplicate assays are shown. (C) Activities of reporter gene constructs in RL1 cells. Luciferase activity is reported relative to pC3HΔNRE-LUC (assigned a value of 1) since pC3H-LUC was not detected in these assays.