FIG. 5.

p53 transactivation activity is functional in Tax-induced tumors and tumor-derived cell lines. Total cellular RNA was isolated from nonirradiated (−) and irradiated (IR) (+) cells at 2 h postirradiation. RT-PCRs were performed using primers specific for p21, bax, mdm2, and p53 mRNA. Levels of GAPDH were measured in parallel to demonstrate that equivalent amounts of RT product were used in each reaction. Cells containing wild-type p53 (204-3-1), mutant p53 (143-2M), and no p53 (L1-2) were utilized as controls to measure p53 transactivation activity.