TABLE 2.
p53 transactivation in Tax-induced tumors
| Cell type | Fold activation Following IRa
|
|||
|---|---|---|---|---|
| p21 | bax | mdm2 | p53 | |
| p53 wild type | 4.5 | 3.5 | 3 | 5 |
| p53 null | 1.1 | 0.9 | 1 | 1.1 |
| p53 mutant | 0.9 | 0.5 | 1 | 1.1 |
| F8 | 4 | 3 | 1.5 | 3 |
| SC | 20 | 4.5 | 1.5 | 2.9 |
| Control spleen | 10 | 3 | 3 | 4 |
| Tax spleen | 4 | 3 | 4 | 3 |
| Tax foot | 10 | 7.5 | 2 | 2 |
a
Total RNA isolated from nonirradiated and irradiated cells was utilized in RT-PCRs with primers specific for p21, bax, mdm2, and p53 mRNA (Fig. 5). Levels of GAPDH were measured in parallel and normalized to calculate the fold activation of each transcript following γ-irradiation (IR). The fold activation averages of three separate experiments are shown.