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. 2024 Sep 6;43(20):4472–4491. doi: 10.1038/s44318-024-00223-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A) QPCR comparison of mRNA levels of truncated Hdc. Total RNA was extracted from heads of Hdc-F (trp-Hdc-mCherry), Hdc-dN (trp-Hdc-dN-mCherry), emc5-Hdc (trp-emc5-Hdc-mCherry), and Syt1-Hdc (trp-Syt1-Hdc-mCherry) flies. A primer pair against mCherry sequences was used for QPCR, and results were normalized to rp49. Hdc-F vs Hdc-dN p = 0.0854, Hdc-F vs emc5-Hdc p = 0.0504, Hdc-F vs Syt1-Hdc p = 0.0557. Dunnett’s one-way ANOVA, n = 3, mean ± sd, ns not significant. (B) Head horizontal sections of trp-Hdc-mCherry flies expressing GFP^RNAi (GMR>GFP^RNAi, trp-Hdc-mCherry GMR-Gal4/UAS-GFP^RNAi), prosα5^RNAi (GMR>prosα5^RNAi, trp-Hdc-mCherry GMR-Gal4/UAS-prosα5^RNAi), and atg7^RNAi (GMR>atg7^RNAi, trp-Hdc-mCherry GMR-Gal4/UAS-atg7^RNAi) were labeled with mCherry (red) and LOVIT (green) antibodies. Scale bars, 20 μm. (C) Quantification of Hdc-mCherry fluorescence ratio between lamina and retina. Three fly head sections were used for quantification. ctr vs prosα5^RNAi ****p < 0.0001, ctr vs atg7^RNAi p = 0.9986. Dunnett’s one-way ANOVA, mean ± sd, ns not significant.