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. 2024 Sep 6;43(20):4472–4491. doi: 10.1038/s44318-024-00223-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Immunostaining of mCherry in trp-hHdc-mCherry and trp-hHdc^K305G-mCherry flies revealed no changes in the distribution and levels of ectopic hHdc proteins. hHdc-mCherry vs hHdc^K305G-mCherry p = 0.1738. Unpaired t-test, n = 3, mean ± sd. (B) Histamine was immunolabeled in horizontal sections of heads of trp-hHdc-mCherry and trp-hHdc^K305G-mCherry flies. Quantification of histamine fluorescence intensity ratios between the entire lamina and retina were based on three sections. hHdc-mCherry vs hHdc^K305G-mCherry **p = 0.0028. Unpaired t-test, mean ± sd. (C) TEM images from wildtype and lovit¹ flies that were 1 (upper) or 20 days old (bottom). Scale bar, 2 μm. Ten sections were used for quantification. wt vs lovit¹ ****p < 0.0001. Unpaired t-test, mean ± sd.