Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 15;15:8887. doi: 10.1038/s41467-024-53277-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 1 — a Normal oocyte lysates, before (Input) or after immunoprecipitation with antibody to TLE6 (top), MATER (middle) or FLOPED (bottom) were immunoblotted and probed with antibodies to pan 14-3-3 and TLE6, MATER, or FLOPED, respectively. IgG, normal immunoglobulin (negative control). b Normal oocytes isolated from normal females were fixed, permeabilized, and incubated with antibodies to pan 14-3-3 and FLOPED (top), MATER (middle), or TLE6 (bottom), and with DAPI to visualize DNA. The oocytes were imaged by confocal microscopy. Scale bar, 10 μm. Colocalization of pan 14-3-3 and FLOPED, MATER, or TLE6 was presented in the merge. c In vitro reconstitution of mouse SCMC-14-3-3γ. The mouse SCMC quaternary complex and 14-3-3γ protein were incubated in lysis buffer. Size-exclusion chromatography (Superose™ 6 Increase 10/300 GL) was performed to separate the SCMC-14-3-3γ (marked in dark red box) and excess 14-3-3γ (marked in dark blue box). The SCMC-14-3-3γ is composed of His-tagged MATER (1–1059 aa), Strep-tagged TLE6 (48–581 aa), Strep-tagged FLOPED (1–164 aa), Strep-tagged FILIA (1–124 aa), and 14-3-3γ (1–247 aa). The column was calibrated with thyroglobulin (669 kDa) and ferritin (440 kDa). d Cryo-EM map of the SCMC-14-3-3γ. 14-3-3γ A and 14-3-3γ B refer to two protomers in 14-3-3γ homodimer. MATER, TLE6, and FLOPED are colored in light blue, salmon, and yellow green, respectively. The homodimer of 14-3-3γ is distinguished by 14-3-3γ A (burlywood), which is proximal to the WD40 repeat domain of TLE6, and 14-3-3γ B (gray), which is close to the leucine-rich repeat domain of MATER. The map was shown at level 0.00679. e Two views of the cartoon presentation of the SCMC-14-3-3γ structure. The color scheme is consistent with panel d. The SCMC_core indicates the core complex consisting of MATER, TLE6, and FLOPED.