Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 15;15:8890. doi: 10.1038/s41467-024-53222-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Representative FACS plots of control (Plk4^F/F) and Mb1^Cre Plk4^F/F mice illustrating the gating of pro/pre B (IgD-IgM −), immature (IgD-IgM +) and mature B cells (IgD+IgM +) in the bone marrow after pregating on CD19 + B220 + cells; from pro/pre gate further gating on pro B (cKit + CD25-) and pre B (cKit-CD25 +) cells; and from pre B further gating on small and large pre B cells via FSC-A and SSC-A. b Bar graphs show bone marrow cellularity and a fraction of B cells, pro/pre B, immature IgM +, and mature B cells. c Bar graphs show a fraction of pro and pre and the ratio of pro to pre B cells within the pro/pre B cell population. d Bar graphs show the fraction of small and large pre B and the ratio of large to small pre B cells within the pre B cell population. e Representative FACS plots of control (Plk4^F/F) and Mb1^Cre Plk4^F/F mice illustrating the gating of splenic B cells (CD19 + B220 +); immature (AA4.1 +) and mature B cells (AA4.1-); from immature B cell gate further gating on transitional 1 (T1, IgM+CD23-) and transitional 2 (T2, IgM+CD23 +) B cells and from mature B cell gate further gating on follicular (FO, CD23 +) and marginal zone (MZ, CD21 + CD23-) B cells; f Bar graphs show spleen cellularity and fraction of B cells, mature and immature B cells in the spleen. g Bar graphs show the fraction of transitional T1 and T2 cells within the population of immature B cells. h Bar graphs show the fraction of follicular (FO) and marginal zone (MZ) B cells within the population of all cells. Error bars in all panels represent mean ± SD. n = 3 biological replicates for both genotypes (individual mice). Compared to the Student’s two-tailed, unpaired t test; Source data are provided as a Source Data file.