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. 2024 Aug 29;43(20):4786–4804. doi: 10.1038/s44318-024-00210-5

Figure EV2. Biophysical validation of iM folding.

Figure EV2

(A) Validation of identified iMs by DNA synthesis and circular dichroism spectroscopy at pH 6.0 and a temperature of 25 °C of selected sequences proximal of promoter regions in known oncogenes. NC1(5’-CAGACTGTCGATGAAGCCCTG-3’) and NC2 (5’-CTAGTTATTGCTCAGCGGTG-3’) negative control sequences. (B) Effects of Temperature (25 °C or 37 °C) and pH (pH 6 or pH 7.4) on CD spectroscopy using identified iM regions associated to the genes HOXC13, SIRPA, TSHR, and the positive control hTelo sequence.