Table 1.
Chloroplast proteins associated with D1 RNCs and functional in chloroplast or thylakoid biogenesis.
| Uniprot ID | Uniprot names | Function | Psat | Potential AT | D1 RNC |
|---|---|---|---|---|---|
| P37107 | Signal recognition particle 54 kDa protein (cpSRP54) | Protein targeting | Psat3g032320.1 | AT5G03940.1 | s, m, l |
| O82230 | Suppressor of tic40 protein 2 (STIC2) | Thylakoid biogenesis | Psat1g022480.1 | AT2G24020.1 | s, m, l |
| Q1KPV0 | Probable transmembrane GTPase FZO-like (FZL) | Thylakoid biogenesis | Psat6g060000.3 | AT1G03160.1 | s, m |
| Q9M394 | Fructokinase-like 1 (FLN1; SCKL1) | Chloroplast biogenesis | Psat7g243520.1 | AT3G54090.1 | s, m, l |
Mass spectrometry analyses of purified TST-D1 RNCs vs. TST-uS2c RNCs showed a D1 specific enrichment of chloroplast biogenesis factors. All listed proteins were exclusively identified in TST-D1 RNCs and are predicted to contain a chloroplast targeting sequence according to Target-P 2.0. Peptide chain length of TST-D1 RNCs: short (s), medium (m), long (l). Detailed MS/MS analysis data are given in the Dataset EV1. Psat annotation was done according to P. sativum database as described in Methods.