Reagents and tools table
| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
| Experimental Models | ||
| Col-0 (A. thaliana) | ABRC | CS60000 |
| stic2-3 (A. thaliana) | European Nottingham Arabidopsis Stock Center | SALK_001500 |
| ffc1-2 (A. thaliana) | Amin et al, 1999 | N/A |
| ffc1-2 stic2-3 (A. thaliana) | This study | N/A |
| Stellar (E. coli) | Takara | 636766 |
| DH5α (E. coli) | Thermo Fischer Scientific | EC0112 |
| XL1blue (E. coli) | Agilent | 200249 |
| Rosetta2 (DE3) pLysS (E. coli) | Novagen | 70956-M |
| BL21 (DE3) pLysS (E. coli) | Novagen | 69451-M |
| Recombinant DNA | ||
| pET-Duet1 | Novagen | 71146-3 |
| pET-Duet1-His-STIC2 (aa 49–182) | This study | N/A |
| pET29b | Novagen | 69872 |
| pET29b-Alb3C-His (aa 350–462) | Ackermann et al, 2021 | N/A |
| pET29b- Alb3C∆III (aa 350–462 lacking aa 386–403) | This study | N/A |
| pET29b-Alb4C-His (aa 334–499) | Ackermann et al, 2021 | N/A |
| pET29b-Alb4C∆III (aa 334–499 lacking aa 397–414) | This study | N/A |
| pET29b-Alb4C-His R339G | This study | N/A |
| pET29b-Alb4C-His F400G | This study | N/A |
| pET29b-Alb4C-His E407G | This study | N/A |
| pET29b-Alb4C-His R410G | This study | N/A |
| pGEX4T3 | Cytiva | 28954552 |
| pGEX4T3-GST-STIC2 | This study | N/A |
| pGEX4T3-GST-STIC2 G111L | This study | N/A |
| pGEX4T3-GST-STIC2 E112A | This study | N/A |
| pGEX4T3-GST-STIC2 K115A | This study | N/A |
| pGEX4T3-GST-STIC2 E131G | This study | N/A |
| pGEX4T3-GST-STIC2 E112A K115A | This study | N/A |
| Antibodies | ||
| Rabbit anti-cpSRP54M | Walter et al, 2015 | N/A |
| Rabbit anti-STIC2 | This study (Davids Biotechnology) | N/A |
| Rabbit anti-FLN1 | PhytoAB | PHY1889S |
| Rabbit anti-FZL | PhytoAB | PHY1890S |
| Rabbit anti-POR | Agrisera | AS05067 |
| Rabbit anti-TIG1 | Ries et al, 2017 | N/A |
| Rabbit anti-uL4 | Agrisera | AS153076 |
| Rabbit anti-uL24 | PhytoAB | PHY0426S |
| Rabbit anti-uS5 | Uniplastomic | AB003 |
| Rabbit anti-Twin-Strep-tag | IBA Liefscience | 2-1509-001 |
| Rabbit anti-SSU (Rbcs) | Agrisera | AS07259 |
| Rabbit anti-Alb3 | Bals et al, 2010 | N/A |
| Rabbit anti-Alb4 | Gerdes et al, 2006 | N/A |
| Rabbit anti-PsbA (D1) | Agrisera | AS05084 |
| Rabbit anti-PsbD (D2) | Agrisera | AS06146 |
| Rabbit anti-PsaA | Agrisera | AS06172 |
| Rabbit anti-PsaB | Agrisera | AS10695 |
| Mouse anti-Actin | Sigma-Aldrich | A0480 |
| Anti-His | Qiagen | 34460 |
| Oligonucleotides and other sequence-based reagents | ||
| cDNA STIC2 | Arabidopsis Biological Resource Center | U13069 |
| PCR primers | This study | Appendix Table S1 |
| Chemicals, Enzymes and other reagents | ||
| PRECISOR High-Fidelity DNA-polymerase | BioCat | N/A |
| Phusion High-Fidelity DNA Polymerase | Thermo Fischer Scientific | F530S |
| BamHI | Thermo Fischer Scientific | ER0051 |
| SalI | Thermo Fischer Scientific | ER0641 |
| T4 DNA ligase | Thermo Fischer Scientific | EL0014 |
| Percoll® | Cytiva | GE17-0891-01 |
| 10x BXT | IBA Lifescience | 2-1042-025 |
| [35S]-methionine | Hartmann Analytics | SRM-01 |
| RiboLock RNase Inhibitor | Thermo Fischer Scientific | EO0382 |
| Nitrocellulose membrane | Macherey-Nagel | 741290 |
| PVDF membrane | Millipore | IPVH00010 |
| SuperSignal™ West Pico PLUS Chemiluminescent Substrate | Thermo Fischer Scientific | 34580 |
| SuperSignal West Femto substrate | Thermo Fischer Scientific | 34094 |
| Bio-Rad Protein Assay Dye Reagent Concentrate | BioRad | 5000006 |
| Software | ||
| ImageJ | https://imagej.net/software/imagej/ | N/A |
| Alphafold-Multimer | Evans et al, 2021; Jumper et al, 2021 | N/A |
| Colabfold 1.5.3 | Mirdita et al, 2022 | N/A |
| MaxQuant 1.6.10.43 | Cox and Mann, 2008 | N/A |
| Other | ||
| InFusion® HD EcoDry | Takara | 638955 |
| QuikChange Lightning Site-directed mutagenesis | Agilent Technologies | 210514 |
| HisTrap HP column, 5 ml | Cytiva | 17524802 |
| Glutathione Sepharose 4B GST-tagged protein purification resin | Cytiva | 17075601 |
| TranscriptAid T7 high yield transcription kit | Thermo Fischer Scientific | K0441 |
| MEGAclear™ transcription clean-up kit | Invitrogen | AM1908 |
| MagStrep® Strep-Tactin® XT beads | IBA Lifescience | 2-5090-002 |
| StrepTrap™ XT column, 1 ml | GE Healthcare | 29-4013-20 |