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. 2024 Jul 3;3(3):39. doi: 10.20517/mrr.2024.21

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© The Author(s) 2024.

© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Experimental Overview and evaluation of bioinformatics workflow. (A) Flowchart depicting mouse fecal sample processing workflow and MS data acquisition. (B) Flowchart depicting different bioinformatic workflows tested in this study. Histograms of the total number of peptides and proteins identified with the different bioinformatics processing workflows as shown in (B) for (C) DDA and (D) DIA datasets, respectively. MS: Mass spectrometry; DDA: data-dependent acquisition; DIA: data-independent acquisition.