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. 2024 Jul 3;3(3):39. doi: 10.20517/mrr.2024.21

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© The Author(s) 2024.

© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Quantitation of small proteins and antimicrobial peptides in mouse fecal samples. (A) Count and (B) relative abundance of small proteins in samples using a 100 amino acid cut-off. Functional enrichment analysis of identified small proteins in (C) the DDA-PASEF dataset and (D) the DIA-PASEF dataset, using all identified proteins as background in DDA and DIA datasets, respectively. Functional annotation was performed using GhostKOALA, and only significantly enriched functions were shown (adjusted P value ≤ 0.05). (E) Count and (F) relative abundance of AMPs when searched against the AMPsphere database. Facet_grid function in ggplot2 was used to generate different sections; DC and DDA groups are on the left side, while NC and DIA on the right side. DDA: Data-dependent acquisition; PASEF: parallel accumulation-serial fragmentation; DIA: data-independent acquisition; AMPs: antimicrobial peptides; DC: differential centrifugation.