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. 2024 Aug 6;4(9):100629. doi: 10.1016/j.xgen.2024.100629

Figure 2.

Figure 2

rRNA variants are co-expressed in individual cells as visualized by in situ sequencing

(A) Graphical illustration of SWITCH-seq pipeline.

(B) Two rounds of representative fluorescent in situ sequencing images of HeLa cells (DAPI staining in blue) are presented for the es39l-probed region. We identified a non-variable base C (magenta) at position 4912. At position 4913, two alternative sequences were revealed: the known reference sequence C (cyan) and the alternate variant U (yellow). Data shown as mean percentage ± SD. n = 4 images.

(C) Representative fluorescent images of HeLa cells (DAPI staining in blue) showcase three highly abundant rRNA variants. The positions of the variants are indicated at the bottom of the images, while the reference (magenta) and alternate (green) alleles are indicated at the top, along with their respective rRNA frequencies. Data shown as mean percentage ± SD. n = 4 images per allele.