Table 4.
List of forward and reverse primers used for specific genes (provided by Thermo Fisher).
| Gene | Fwd sequence | Rev sequence |
|---|---|---|
| GAPDH | AGGTCGGTGTGAACGGATT | TGTAGACCATGTAGTTGAGG |
| SOX9 | GACTTCCGCGACGTGGAC | GTTGGGCGGCAGGTACTG |
| COL2A1 | CGCAATAGCAGGTTCACGTACA | CGATAACAGTCTTGCCCCACTT |
| Aggrecan | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGTGTAGAGA |
| COL1A1 | TCTGCGACAACGGCAAGGTG | GACGCCGGTGGTTTCTTGGT |
| COL10A1 | CAAGGCACCATCTCCAGGAA | AAAGGGTATTTGTGGCAGCATATT |
| N‐cadherin | CGAGCCGCCTGCGCTGCCAC | CGCTGCTCTCCGCTCCCCGC |
| β‐catenin | TGGATGGGCTGCCTCCAGGTGAC | ACCAGCCCACCCCTCGAGCCC |
| Integrin β1 | GTGCAATGAAGGGCGTGTT | GTTGCACTCACACACACGACA |
| Integrin β3 | ACACTGGCAAGGATGCAGTGAATTGTAC | CGTGATATTGGTGAAGGTAGACGTGGC |
| Piezo1 | TCGCTGGTCTACCTGCTCTT | GGCCTGTGTGACCTTGGA |