FIG. 3.

Binding of ¹²⁵I-labeled SA11 rotavirus to pure nonacid glycosphingolipids on TLC plates. Glycosphingolipids were separated on TLC plates using chloroform-methanol-water (60:35:8 by volume). One chromatogram (A) was stained with anisaldehyde. Other chromatograms were incubated with the following purified radiolabeled viral particles: TLP from SA11 (B), DLP from SA11 (C), TLP from NCDV (D), and DLP from NCDV (E). Each lane contained 2 μg of pure glycosphingolipids. Lanes 1, GA1; lanes 2, globoside; lanes 3, GA2; lanes 4, lactosylceramide t18:0-h16:0-h24:0 (Galβ4Glcβ1Cer); lanes 5, isoglobotriaosylceramide; lanes 6, lactotetraosylceramide; lanes 7, neolactotetraosylceramide; lanes 8, B5 glycosphingolipid.