FIG. 4.

Binding of ¹²⁵I-labeled SA11 rotavirus to mixtures of acid glycosphingolipids on TLC plates. Glycosphingolipids were separated on TLC plates using chloroform-methanol-water (60:35:8 by volume). One chromatogram (A) was stained with anisaldehyde. Other chromatograms were incubated with the following purified radiolabeled viral particles: TLP from SA11 (B) and DLP from SA11 (C). Each lane contained 40 μg of glycosphingolipid mixture. Lanes 1, nonacid glycosphingolipids of human erythrocytes, blood group AB; lanes 2, nonacid glycosphingolipids of guinea pig erythrocytes; lanes 3, acid glycosphingolipids of human meconium; lanes 4, acid glycosphingolipids of adult pig intestine; lanes 5, acid glycosphingolipids of newborn pig intestine; lanes 6, acid glycosphingolipids of the proximal part of calf small intestine; lanes 7, acid glycosphingolipids of the middle part of calf small intestine; lanes 8, acid glycosphingolipids of the distal part of calf small intestine.