FIG. 9.

Comparison of the binding of ¹²⁵I-labeled NCDV rotavirus to acid glycosphingolipids of newborn and adult bovine small intestinal epithelia. Glycosphingolipids were separated on TLC plates using chloroform–methanol–0.25% KCl in water (50:40:10, by volume). One chromatogram (A) was stained with anisaldehyde. Other chromatograms were incubated with the following purified radiolabeled viral particles: TLP from NCDV (B) and DLP from NCDV (C). Each lane contained 2 μg of purified glycosphingolipids or 40 μg of glycosphingolipid mixture. Lanes 1, acid fraction of newborn calf small intestinal epithelium; lanes 2, NeuGc-GD1a; lanes 3, GA2; lanes 4, NeuAc-GM3; lanes 5, NeuGc-GM3; lanes 6, NeuGc-GM1; lanes 7, NeuGc-GD2; lanes 8, acid fraction of adult bovine small intestinal epithelium.