TABLE 1.
Binding of 125I-labeled NCDV and SA11 rotavirus strains to glycosphingolipids on thin-layer plates
| Trivial name | Structure | Binding on TLC platesa
|
|||
|---|---|---|---|---|---|
| DLP (SA11, NCDV, and UK) | TLP
|
||||
| SA11 | NCDV | UK | |||
| 1. Galactosylceramide | Galβ1Cer | − | − | − | − |
| 2. Glucosylceramide | Glcβ1Cer | − | − | − | − |
| 3. LacCer d18:1-16:0/24:1 | Galβ4Glcβ1Cer | − | − | − | − |
| 4. LacCer t18:0-h16:0-h24:0 | Galβ4Glcβ1Cer | + | + | + | + |
| 5. Isoglobotriaosylceramide | Galα3Galβ4Glcβ1Cer | − | − | − | ND |
| 6. Globoside | GalNAcβ3Galα4Galβ4Glcβ1Cer | − | − | − | − |
| 7. GA2 | GalNAcβ4Galβ4Glcβ1Cer | + | + | + | + |
| 8. GA1 | Galβ3GalNAcβ4Galβ4Glcβ1Cer | ++ | ++ | ++ | ++ |
| 9. Lactotetraosylceramide | Galβ3GlcNAcβ3Galβ4Glcβ1Cer | + | + | + | ND |
| 10. Neolactotetraosylceramide | Galβ4GlcNAcβ3Galβ4Glcβ1Cer | + | + | + | ND |
| 11. B5 | Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | − | − | ND |
| 12. NeuAc-GM3 | NeuAcα3Galβ4Glcβ1Cer | − | − | − | + |
| 13. NeuGc-GM3 | NeuGcα3Galβ4Glcβ1Cer | − | ++ | ++ | − |
| 14. NeuAc-GM2 | GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | + | + | ND |
| 15. NeuGc-GM2 | GalNAcβ4(NeuGcα3)Galβ4Glcβ1Cer | − | + | + | + |
| 16. NeuAc-GM1a | Galβ3GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | − | − | + |
| 17. NeuGc-GM1a | Galβ3GalNAcβ4(NeuGcα3)Galβ4Glcβ1Cer | − | − | − | + |
| 18. NeuAc-GD1a | NeuAcα3Galβ3GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | ± | ± | ND |
| 19. NeuGc/Ac-GD1a | NeuGcα3Galβ3GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | + | + | + |
| 20. NeuGc-GD1a | NeuGcα3Galβ3GalNAcβ4(NeuGcα3)Galβ4Glcβ1Cer | − | + | + | ND |
| 21. NeuAc-GD1b | Galβ3GalNAcβ4(NeuAcα8NeuAcα3)Galβ4Glcβ1Cer | − | − | − | ND |
| 22. NeuAc-GT1b | NeuAcα3Galβ3GalNAcβ4(NeuAcα8NeuAcα3)Galβ4Glcβ1Cer | − | − | − | ND |
| 23. NeuAcα3-neolactotetraosylceramide | NeuAcα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | + | + | + |
| 24. NeuAcα6-neolactotetraosylceramide | NeuAcα6Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | ± | ND | ND |
| 25. NeuAc-SLex | NeuAcα3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer | − | + | + | ND |
| 26. NeuAc-SLea | NeuAcα3Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | − | − | ± | ND |
| 27. NeuGc-GD2 | GalNAcβ4(NeuGcα8NeuGcα3)Galβ4Glcβ1Cer | − | ND | − | ND |
| 28. NeuAc-GD3 | NeuAcα8NeuAcα3Galβ4Glcβ1Cer | − | − | − | ND |
| 29. NeuAc-GQ1b | NeuAcα8NeuAcα3Galβ3GalNAcβ4(NeuAcα8NeuAcα3)Galβ4Glcβ1Cer | − | − | − | ND |
| 30. None | NeuGcα3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | ++ | ND | ND |
| 31. None | Galβ4GlcNAcβ6(NeuAcα6Galβ4GlcNAcβ3)Galβ4Glcβ1Cer | − | − | − | ND |
| 32. None | Galα3Galβ4GlcNAcβ6(NeuAcα3Galβ4GlcNAcβ3)Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | + | + | ND |
a
++, +, and ±, strong, moderate, and weak darkening, respectively, of the autoradiogram when 2 μg of the glycosphingolipid was applied on the thin-layer plate; −, no binding at all. ND, binding was not determined.