FIG. 8.
Timing of reverse transcription in Molt4-Clone8 cells infected with vector pseudotypes. A total of 106 Molt4-Clone8 cells in six-well plates were infected with 105 GFU of V1EGFP pseudotyped with different SIVmac envelopes. A 100 μM concentration of PMPA was added at the times indicated, and the numbers of GFP-positive cells were measured at 4 days postinfection by flow cytometry. Infection levels are shown as percentage of GFP-positive cells in the absence of PMPA.