Figure 6.

Gene‐edited GMEC alleviates inflammation and TJ damage by high expression of LYZ. a) Flowchart of GMEC organoid establishment. b) GMEC organoid culture 3, 6, 9 and 12 d status. Scale bar: 100 µm. c) CD49f staining of GMEC organoids was performed using the IF method. Scale bar: 100 µm. d–g) Analysis of LYZ, IL‐6, TNFα, IL‐1β protein expression. h) Primary GMEC and gene‐edited GMEC were treated with 5 and 10 µg mL⁻¹ LPS for 12 h, and then the culture medium was replaced for 12 h to detect the protein expression level of TLR4, MyD88, p‐IKBα, IKBα, p‐NF‐κB‐p65, NF‐κB‐p65, ZO‐1 and Occludin. i) The protein expression of p‐NF‐κB‐p65 in the nucleus was detected by IF. Scale bar: 20 µm. j) The protein expression of p‐NF‐κB p65 in GMEC organoids was detected by IF. Scale bar: 100 µm. Values are expressed as mean ± SEM (n = 3 per group) by one‐way ANOVA. *: indicates significant difference (P < 0.05), **: indicates that the difference is highly significant (P < 0.01) and ns: indicates no significant difference (P > 0.05).