TABLE 1.
Spectrum of activitya
| Organism | DHB MIC (μg mL−1) |
|---|---|
| Pathogenic bacteria | |
| Escherichia coli MG1655 | 1 |
| Escherichia coli ATCC 25922 | 1–2 |
| Escherichia coli WO153 asmB1, ΔtolC | 1 |
| Enterobacter cloacae ATCC 13047 | 0.5 |
| Serratia marcescens ATCC 13880 | 1–2 |
| Klebsiella pneumoniae BAA-2146 | 2 |
| Klebsiella pneumoniae BAA-43816 | 4 |
| Proteus mirabilis HI4320 | 2–8 |
| Acinetobacter baumannii ATCC 19606 | 16 |
| Pseudomonas aeruginosa PAO1 | >128 |
| Staphylococcus aureus HG003 | >128 |
| Gut anaerobes | |
| Bacteroides uniformis KLE 1601 | >128 |
| Eggerthella lenta KLE 2234 | >128 |
| Bifidobacterium bifidum KLE 2535 | >128 |
| Veillonella ratti KLE 2366 | 64 |
| Ruminococcus gnavus ATCC 29149 | >128 |
| Clostridium hathewayi KLE 1709 | >128 |
| Enterococcus faecalis KLE 2341 | >128 |
| Human cell lines | |
| HepG2 | >128 |
| FaDu | >128 |
| HEK293 | >128 |
a
Bacteria were cultured in Mueller-Hinton II broth, and minimum inhibitory concentration (MIC) was determined in duplicate by broth microdilution in microtiter plates. For anaerobes, bacteria were cultured in duplicate in Gifu Anaerobic Broth in an anaerobic chamber. For cytotoxicity, cells were grown in triplicate in Eagle’s minimum essential media, and viability was determined by resazurin cell viability assay.