Fig. 2.

Metastasis of HSC-3 cells pretreated with bacterial extracellular vesicles (EVs; 10 µg/ml) from: A. actinomycetemcomitans D7SS-WT (wild-type), D7SS-cdt (lacking the cytolethal distending toxin, CDT), SA3138-WT (wild-type), SA3139-LPS-O (lacking the lipopolysaccharide (LPS) O-antigen), and P. gingivalis (wild-type). a Metastasis rate was not significantly affected by pretreatment with EVs from D7SS-WT and D7SS-cdt strains. b HSC-3 cells pretreated with SA3139-LPS-O-derived EVs had significantly lower metastatic rate than control cells. c No statistically significant changes were seen in the metastasis rates of HSC-3 cells pretreated with P. gingivalis EVs compared to controls. * p < 0.05. Values are shown as mean values from each experiment and line at mean. All experiments were repeated independently three times