Fig. 3.

Preferential dissemination sites of the metastatic tumor cells in zebrafish larvae. HSC-3 cells were treated with bacterial extracellular vesicles (EVs; 10 µg/ml) from: A. actinomycetemcomitans D7SS-WT (wild-type), D7SS-cdt (lacking the cytolethal distending toxin, CDT), SA3138-WT (wild-type), SA3139-LPS-O (lacking the lipopolysaccharide (LPS) O-antigen), and P. gingivalis (wild-type). A trend of lower metastasis rate to both head and tail was noted in cells pretreated with EVs from a A. actinomycetemcomitans D7SS-cdt, b A. actinomycetemcomitans SA3139-LPS-O and c P. gingivalis, although the differences were not statistically significant. d Demonstrative images of metastasis in head and tail of zebrafish larvae. Red areas represent tumor cells. Values are shown as mean ± SD. All experiments were repeated independently three times