FIGURE 2:

msYFP2 is photostable in live yeast cells. Golgi cisternae were labeled by using gene replacement to fuse the indicated YFPs to Sec7. Confocal z-stacks were captured every 2 s for 5 min and then average projected to create movies. (A) Images from the first frames of representative movies. Scale bar, 2 μm. (B) Plots of averaged fluorescence signals. For each strain, three movies were captured with ∼10–15 cells in each movie. Averaged fluorescence signals were normalized to the values at time zero, and the data were fit to exponential decay curves. The bars represent SEM values. The rate constant for photobleaching of mEYFP was about twice that for the other two YFPs.