FIGURE 3:
msYFP2 is photostable in Drosophila larvae. (A) Simplified diagram of Yki regulation. Wts inhibits the translocation of Yki from the cytoplasm into the nucleus, so in the absence of Wts, Yki enters the nucleus. (B) Depletion of Wts in the posterior compartment of the wing imaginal disk using the hh>Gal4 driver (mChNLS positive side) leads to significant nuclear accumulation of Yki-msYFP2 compared with the control anterior compartment (mChNLS negative side), suggesting that Yki-msYFP2 behaves normally in vivo. Scale bar, 20 μm. (C) Plot of Yki-msYFP2 and Yki-Venus photobleaching during scanning confocal imaging of the third instar wing imaginal epithelium. For each Yki fusion, data from five experiments were averaged to generate the curve, which was normalized to the average value from the first scan. Bars representing SEM values are plotted but are too small to be seen.