FIGURE 4:

msYFP2 is photostable in cultured mammalian cells. (A) Labeling of the Golgi ribbon in RPE-1 cells by transient expression of constructs encoding msYFP2 or mGold fused to GalNAc-T2. Scale bar, 5 μm. (B) Photostability of msYFP2 and mGold fused to GalNAc-T2 in live RPE-1 cells. Five cells from each sample were imaged by capturing confocal z-stacks every 5 s for 5 min, and the signals from the Golgi structures were quantified. Averaged fluorescence signals were normalized to the values at time zero. The bars represent SEM values.