FIGURE 7:

moxGFP2 is suitable for use as a fluorescent tag in the mammalian secretory pathway. (A) Labeling of the Golgi ribbon in RPE-1 cells by transient expression of constructs encoding moxGFP2 or msGFP2 fused to GalNAc-T2. Scale bar, 5 μm. (B) Photostability of moxGFP2 and msGFP2 fused to GalNAc-T2 in live RPE-1 cells. Four cells from each sample were imaged by capturing confocal z-stacks every 5 s for 5 min, and the signals from the Golgi structures were quantified. Mean fluorescence signals were normalized to the values at time zero. The bars represent SEM values.