FIG. 4.
Expression of vIRF-2 rescues IFN-induced block of viral protein synthesis. (A) Autoradiography analysis of metabolically labeled cells transfected with empty (pcDNA3.1) or FLAG/vIRF-2-expressing (vIRF-2F/pcDNA3.1) vector. Cells were mock infected (−) or infected with VSV (+) and 5 h later pulse-labeled with 35S-labeled amino acid mixture as described in Materials and Methods. Positions of early VSV proteins are marked on the right. (B) Relative levels of 29-kDa VSV matrix protein (M) in pcDNA- or vIRF-2F/pcDNA-transfected cells and its dependency on increased concentrations of IFN. Amounts of viral proteins in cells pretreated with increased concentrations of IFN were quantified from autoradiograms by using a PhosphorImager densitometer and data analysis software. Error bars represent standard errors from three independent experiments. (C) Immunoblot detection of FLAG-tagged vIRF-2 in representative samples of pcDNA- or vIRF-2F/pcDNA-transfected cells by using anti-FLAG antibody.