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. 2024 Sep 18;11(9):240610. doi: 10.1098/rsos.240610

Table 2.

The top five most abundant proteins (by peptide count) preserved in foodcrust and ceramic samples after cooking and burial for six months. Data from replicates have been merged. Probable protein source is noted, including where LCA matches to input ingredient, where protein LCA is non-specific and could potentially belong to either input ingredient or another taxonomy, and where protein LCA could not possibly match to the input ingredient and is therefore derived from contamination. This table is based on proteins with the highest peptide count regardless of taxonomic specificity. We note that many muscle proteins are highly conserved; therefore, in electronic supplementary material, S8, we have noted the most abundant proteins (by peptide count) which match to taxonomic family (Salmonidae, Fagaceae or Cervidae) or lower detected in buried foodcrusts.

protein peptide count unipept LCA probable protein source
chestnut foodcrust TATA box binding protein-associated factor (TAF) histone-like fold domain-containing protein 8 root non-specific
actin, alpha cardiac muscle 1 6 Eukaryota non-specific
cupin type-1 domain-containing protein 6 Fagaceae and Q. lobata input ingredient
heat shock protein 70 5 root non-specific
IF rod domain-containing protein 5 Mammalia contaminant
ceramic fast myotomal muscle troponin-T 5 Neopterygii contaminant
IF rod domain-containing protein 3 Mammalia contaminant
keratin, type II cytoskeletal 5 2 Mammalia contaminant
deer foodcrust actin, alpha cardiac muscle 1 29 Eukaryota non-specific
globin family profile domain-containing protein 25 Cervidae input ingredient
TATA box binding protein associated factor (TAF) histone-like fold domain-containing protein 12 root non-specific
elongation factor 1-alpha 11 root non-specific
myoglobin 11 Pecora input ingredient
ceramic myosin-1 6 Craniata non-specific
actin, alpha cardiac muscle 1 5 root non-specific
pyruvate kinase 4 Mammalia non-specific
creatine kinase 2 Craniata non-specific
filamin-C 2 Craniata non-specific
ʟ-lactate dehydrogenase 2 Neopterygii contaminant
myosin binding protein C1 2 Pecora input ingredient
non-selective voltage-gated ion channel VDAC1 2 Mammalia non-specific
salmon foodcrust glyceraldehyde-3-phosphate dehydrogenase 71 Salmonidae input ingredient
myosin heavy chain, fast skeletal muscle-like 56 Salmoninae input ingredient
fast myotomal muscle tropomyosin (Tropomyosin alpha-1 chain) 46 Salmonidae input ingredient
phosphopyruvate hydratase (2-phospho-ᴅ-glycerate hydrolyase) 46 Salmonidae input ingredient
fructose-bisphosphate aldolase 32 Salmonidae input ingredient
ceramic IF rod domain-containing protein 6 Mammalia contaminant
collagen alpha-2(I) chain 3 Artiodactyla contaminant
keratin, type II cytoskeletal 5 3 Sarcopterygii non-specific
keratin, type II cytoskeletal 6A 2 Sarcopterygii non-specific