Table 2.
The top five most abundant proteins (by peptide count) preserved in foodcrust and ceramic samples after cooking and burial for six months. Data from replicates have been merged. Probable protein source is noted, including where LCA matches to input ingredient, where protein LCA is non-specific and could potentially belong to either input ingredient or another taxonomy, and where protein LCA could not possibly match to the input ingredient and is therefore derived from contamination. This table is based on proteins with the highest peptide count regardless of taxonomic specificity. We note that many muscle proteins are highly conserved; therefore, in electronic supplementary material, S8, we have noted the most abundant proteins (by peptide count) which match to taxonomic family (Salmonidae, Fagaceae or Cervidae) or lower detected in buried foodcrusts.
| protein | peptide count | unipept LCA | probable protein source | ||
|---|---|---|---|---|---|
| chestnut | foodcrust | TATA box binding protein-associated factor (TAF) histone-like fold domain-containing protein | 8 | root | non-specific |
| actin, alpha cardiac muscle 1 | 6 | Eukaryota | non-specific | ||
| cupin type-1 domain-containing protein | 6 | Fagaceae and Q. lobata | input ingredient | ||
| heat shock protein 70 | 5 | root | non-specific | ||
| IF rod domain-containing protein | 5 | Mammalia | contaminant | ||
| ceramic | fast myotomal muscle troponin-T | 5 | Neopterygii | contaminant | |
| IF rod domain-containing protein | 3 | Mammalia | contaminant | ||
| keratin, type II cytoskeletal 5 | 2 | Mammalia | contaminant | ||
| deer | foodcrust | actin, alpha cardiac muscle 1 | 29 | Eukaryota | non-specific |
| globin family profile domain-containing protein | 25 | Cervidae | input ingredient | ||
| TATA box binding protein associated factor (TAF) histone-like fold domain-containing protein | 12 | root | non-specific | ||
| elongation factor 1-alpha | 11 | root | non-specific | ||
| myoglobin | 11 | Pecora | input ingredient | ||
| ceramic | myosin-1 | 6 | Craniata | non-specific | |
| actin, alpha cardiac muscle 1 | 5 | root | non-specific | ||
| pyruvate kinase | 4 | Mammalia | non-specific | ||
| creatine kinase | 2 | Craniata | non-specific | ||
| filamin-C | 2 | Craniata | non-specific | ||
| ʟ-lactate dehydrogenase | 2 | Neopterygii | contaminant | ||
| myosin binding protein C1 | 2 | Pecora | input ingredient | ||
| non-selective voltage-gated ion channel VDAC1 | 2 | Mammalia | non-specific | ||
| salmon | foodcrust | glyceraldehyde-3-phosphate dehydrogenase | 71 | Salmonidae | input ingredient |
| myosin heavy chain, fast skeletal muscle-like | 56 | Salmoninae | input ingredient | ||
| fast myotomal muscle tropomyosin (Tropomyosin alpha-1 chain) | 46 | Salmonidae | input ingredient | ||
| phosphopyruvate hydratase (2-phospho-ᴅ-glycerate hydrolyase) | 46 | Salmonidae | input ingredient | ||
| fructose-bisphosphate aldolase | 32 | Salmonidae | input ingredient | ||
| ceramic | IF rod domain-containing protein | 6 | Mammalia | contaminant | |
| collagen alpha-2(I) chain | 3 | Artiodactyla | contaminant | ||
| keratin, type II cytoskeletal 5 | 3 | Sarcopterygii | non-specific | ||
| keratin, type II cytoskeletal 6A | 2 | Sarcopterygii | non-specific |