FIG. 2.

Plaque morphology and growth kinetics of wild-type and recombinant coxsackieviruses. (A) Plaque assays of wtCVB (strain H3) (28) and rCVB3.1, rCVB3.2, and rCVB3.3 were performed in parallel on HeLa cell monolayers. The numbers indicate the average plaque diameter of 40 to 60 individual plaques ± the standard deviation. (B) A one-step growth curve was generated to compare the in vitro growth kinetics of rCVB3 and wtCVB. HeLa cell monolayers were infected at an MOI of 10, and the amount of infectious virus was determined at each time point by a plaque assay. p.i., postinfection.