Table 6.
Summary of female mouse echocardiography values in control and HFD studies
| Echo Parameter | Young |
Aged |
||||||
|---|---|---|---|---|---|---|---|---|
| WT CD | SSPN−/− CD | WT HFD | SSPN−/− HFD | WT CD | SSPN−/− CD | WT HFD | SSPN−/− HFD | |
| n | 7 | 5 | 6 | 7 | 7 | 18 | 6 | 8 |
| HR, beats/min | 461.7 ± 21.1 | 429.9 ± 10.4 | 460.92 ± 11.0 | 445.43 ± 19.7 | 531.8 ± 34.4 | 477.0 ± 12.0 | 509.5 ± 22.0 | 482.2 ± 10.2 |
| LVID;s, mm | 2.23 ± 0.16 | 2.40 ± 0.22 | 1.80 ± 0.08 | 1.85 ± 0.04 | 1.83 ± 0.20 | 1.95 ± 0.06 | 1.44 ± 0.15 | 1.83 ± 0.09 |
| LVID;d, mm | 3.44 ± 0.17 | 3.56 ± 0.22 | 3.47 ± 0.18 | 3.28 ± 0.07 | 3.20 ± 0.17 | 3.33 ± 0.06 | 2.88 ± 0.10a | 3.34 ± 0.06 |
| LV;s, µL | 11.62 ± 1.15 | 14.21 ± 1.97 | 16.27 ± 2.16 | 8.96 ± 1.98 | 13.36 ± 3.14 | 12.92 ± 1.15 | 6.89 ± 2.39 | 15.14 ± 1.99 |
| LV;d, µL | 44.53 ± 3.51 | 48.82 ± 1.65 | 55.06 ± 5.12 | 40.34 ± 2.65 | 41.83 ± 4.86 | 45.68 ± 1.85 | 32.59 ± 3.17a | 45.63 ± 1.88 |
| LV EF, % | 61.74 ± 2.89 | 62.09 ± 3.76 | 70.61 ± 2.56 | 78.18 ± 2.30c | 74.98 ± 4.09 | 72.99 ± 1.35 | 82.40 ± 3.30 | 76.6 ± 2.57 |
| LV FS, % | 32.6 ± 1.98 | 32.99 ± 2.60 | 39.39 ± 2.16 | 46.31 ± 2.37c | 39.64 ± 2.80 | 41.47 ± 1.12 | 50.88 ± 3.26 | 45.3 ± 1.96 |
| SV, µL | 30.41 ± 3.10 | 32.97 ± 3.84 | 36.06 ± 4.71 | 36.59 ± 3.32 | 32.29 ± 2.93 | 32.79 ± 1.01 | 25.71 ± 0.95 | 35.1 ± 0.98 |
| CO, µL | 12.64 ± 2.17 | 14.30 ± 1.83 | 16.61 ± 2.30 | 14.58 ± 2.07 | 16.18 ± 2.00 | 14.77 ± 1.02 | 12.94 ± 0.71 | 17.0 ± 0.71 |
| MV E/A | 1.97 ± 0.26 | 1.62 ± 0.11 | 2.28 ± 0.19 | 2.27 ± 0.13 | 1.46 ± 0.10 | 1.31 ± 0.19 | 1.34 ± 0.14 | 1.33 ± 0.09b |
| IVRT, ms | 16.05 ± 0.75 | 13.92 ± 1.10 | 18.31 ± 1.95 | 18.17 ± 1.88 | 15.17 ± 1.23 | 15.70 ± 1.46 | 14.10 ± 1.04 | 15.31 ± 0.70 |
| IVCT, ms | 13.10 ± 1.20 | 11.85 ± 1.11 | 11.19 ± 0.01 | 11.97 ± 0.01 | 16.18 ± 2.50 | 10.41 ± 0.56 | 13.37 ± 2.18 | 16.42 ± 1.12 |
| LVmass, mg | 103.0 ± 13.16 | 103.5 ± 4.70 | 108.4 ± 9.51 | 107.6 ± 5.74 | 104.6 ± 13.13 | 109.4 ± 6.60 | 116.1 ± 22.92 | 119.1 ± 13.45 |
| Age, mo | 8.41 ± 0.40 | 8.35 ± 0.50 | 7.74 ± 0.25 | 8.12 ± 0.51 | 17.43 ± 0.55 | 17.20 ± 0.15 | 18.69 ± 0.40 | 17.17 ± 0.19 |
| HW/TL, mg/mm | 7.19 ± 0.21 | 6.62 ± 0.42 | 7.77 ± 0.17 | 7.08 ± 0.19 | 6.25 ± 0.68 | 6.34 ± 0.84 | 7.06 ± 1.39 | 6.19 ± 0.41 |
Values are means ± SE; n, number of mice. CD, control diet; CO, cardiac output; Echo, echocardiography; EF, ejection fraction; FS, fractional shortening; HFD, high-fat diet; HR, heart rate; HW/TL, heart weight-to-tibia length ratio; ID;d, inner dimension, diastole; ID;s, inner dimension, systole; IVCT, intraventricular contraction time; IVRT, isovolumic relaxation time measurements; LV, left ventricular; SV, stroke volume; MV E/A, mitral valve E/A; SSPN−/−, sarcospan deficient; WT, wild type. One-way ANOVA was used to compare differences between groups. aP < 0.05, WT (young vs. aged) HFD mice; bP < 0.05, SSPN−/− (young vs. aged) HFD mice; cP < 0.05, SSPN−/− (young) CD vs. HFD mice. Significant groups: WT (young vs. aged) HFD mice: *LVID;s, *LV;d; SSPN−/− (young vs. aged) HFD mice: *E/A, IVRT; SSPN−/− (young) CD vs. HFD mice: *LV EF%, *LV FS%), **; WT vs. SSPN−/− aged CD mice, WT (aged) CD vs. HFD mice.