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. 2024 Oct 16;10(42):eadi6927. doi: 10.1126/sciadv.adi6927

Fig. 1. MYH7-variant CMTs exhibit increased collagen content, leading to hypocontractility.

Fig. 1.

(A) Schematic of CMT made with hiPSC-CMs and vCFs in a fibrin-based gel. (B) Representative images of maximum intensity z-projections of CMTs fixed and stained for collagen type I on day 7. Scale bar, 100 μm. (C) Mean fluorescence intensity of collagen type I in CMTs: WT (n = 35), R403Q+/− (n = 37) (N = 3). (D) Schematic of nanoindentation of CMT and average load-displacement curves from nanoindentation of CMTs. (E) Quantification of the elastic modulus: WT (n = 7), R403Q+/− (n = 8) (N = 3). (F) Representative bright-field images of WT (left) and R403Q+/− (right) CMTs with representative contractile stress traces overtime. Scale bar, 150 μm. (G) Contractile stress generation in CMTs: WT (n = 65), R403Q+/− (n = 73) (N = 4). (H) Contractile relaxation rate of CMTs: WT (n = 65), R403Q+/− (n = 73) (N = 4). (I) Mean fluorescence intensity of collagen type I in CMTs made with mitomycin-C (Mito-C)–treated vCFs (n > 15) (N = 2). (J) Contractile stress of CMTs made with Mito-C–treated vCFs (n > 37) (N = 4). Individual CMTs (n) across all independent experiments (N) are shown with means ± SEM.